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Provedor de dados:  OAK
País:  Japan
Título:  Alfalfa-Verticillium Albo-atrum Interactions : In Vitro Responses of Alfalfa Callus to Fungal Culture Filtrate and Cell Wall Components
Autores:  Koike, Masanori
Nanbu, Kouhei
Shimada, Tohru
Data:  1992-01-31
Ano:  1992
Palavras-chave:  Verticillium albo-atrum
Alfalfa
Callus
Cell wall components Culture filtrates
Disease resistance
Resumo:  日本草地学会Journal of Japanese Society of Grassland Science 37(4):412-419(1992)より転載

Interactions between alfalfa and Verticillium albo-atrum were examined in terms of the following aspects:growth of V. albo-atrum on callus;growth of callus on the medium containing culture filtrate of V. albo-atrum and production of antifungal substances in callus treated with cell wall components (elicitor) from V. albo-atrum. Mycelial growth of V. albo-atrum was likely to be suppressed on the callus from resistant cultivars (Vertus and 5444), accompanied by strong browning. The growth of callus from Vertus (resistant, R) and Kitawakaba (susceptible, S) were vigorous on the medium containing 0.5 or 1% culture filtrate, while those from 5444 (R) and Europe (S) were inhibited. Antifungal substances were detected in all cultivars treated with cell wall components, with concentrations being higher in resistant than in susceptible cultivars. These results suggest that antifungal activity in the callus extracts indicated by the inhibition of germ tube elongation of fungal conidia is the best criterion for the selection of resistant cultivars.
Idioma:  Inglês
Identificador:  http://ir.obihiro.ac.jp/dspace/handle/10322/1772
Editor:  Japanese Society of Grassland Science
Formato:  application/pdf
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